Improved HPLC method for total plasma homocysteine detection and quantification.

Improved HPLC method for total plasma homocysteine detection and quantification.

Recent clinical research has pointed at hyperhomocysteinemia as an independent risk factor in a number of cardiovascular and neurological diseases. We have improved a chromatographic method of total plasma homocysteine measurements in order to obtain higher sensitivity, reliability and reproducibility. The method demonstrates excellent linearity (R=0.999), range (<2-100 microM), precision (inst...

Regular paper Improved HPLC method for total plasma homocysteine detection and quantification

Recent clinical research has pointed at hyperhomocysteinemia as an independent risk factor in a number of cardiovascular and neurological diseases. We have improved a chromatographic method of total plasma homocysteine measurements in order to obtain higher sensitivity, reliability and reproducibility. The method demonstrates excellent linearity (R = 0.999), range (< 2–100 μM), precision (instr...

Measurement of Plasma Total Homocysteine by HPLC

23 mg/L for the evaluation tube, with an average difference between tube types of 35 6 4.3 mg/L. The test results for blood lead and EP on samples collected in the evaluation tubes were clearly equivalent to those collected in the independently certified, lead-free control tubes. The availability of pediatric pretested lead-free collection tubes will contribute to accurate and reliable diagnosi...

Rapid and accurate HPLC assay for plasma total homocysteine and cysteine in a clinical laboratory setting.

Method comparison for total plasma homocysteine between the Abbott IMx analyzer and an HPLC assay with internal standardization.

for clinical laboratory use. The method is simple and precise, enabling comparatively easy processing of large numbers of specimens. The performance characteristics of the method presented here are comparable with those utilizing fluorescence detection. The major advantage of this method is the avoidance of derivatization. The use of penicillamine as an internal standard for HCySH analysis had ...